Part:BBa_K3631000

Part A of Serotonin Whole Cell Biosensor

This part consists tac Promoter and lasR ORF. It serves to express lasR protein, which is an important signaling molecule in lasR-lasI signaling system in Pseudomonas aeruginosa. It could be used to design a genomic circuit or biosensor.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 157
  Illegal PstI site found at 565
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 157
  Illegal PstI site found at 565
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 85
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 157
  Illegal PstI site found at 565
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 157
  Illegal PstI site found at 565
  Illegal NgoMIV site found at 412
  Illegal AgeI site found at 609
  
• 1000
  COMPATIBLE WITH RFC[1000]